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| 产品名称： | pMR102 |
|---|---|
| 商品货号： | TS150957 |
| Designations： | pMR102 |
| Depositors： | L Regan, M Munson |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0999999046325680 Vector: pMR102 (phagemid) Promoters: Promoter for expression T7 (phi10) Construction: pMR101 Marker(s):kanR Construct size (kb): 4.099999904632568 Features: marker(s): kanR operator: lac promoter for expression: T7 (phi10) replicon: M13 replicon: p15A |
| Applications： | expression vector vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRV--4.1; BstEII/PstI--3.0, 1.1. Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell. If used in an Escherichia coli strain that expresses T7 polymerase under the control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result in high levels of recombinant protein production. Use of 5NcoI and 3BamHI cloning sites is similar to that of other expression systems, which facilitiates transfer of genes into these pMR vectors. Differs from pMR101 (ATCC 87115) by the removal of BglII and Xba sites. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Munson M, et al. ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene 144: 59-62, 1994. PubMed: 8026759 |
| Shipped： | freeze-dried |