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| 产品名称： | pTZ19RBS.K |
|---|---|
| 商品货号： | TS151080 |
| Designations： | pTZ19RBS.K |
| Depositors： | O De Backer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.8959999084472660 Vector: pTZ19RBS.K (phagemid) Promoters: Promoter T7 (phi10) Construction: pTZ19R Marker(s):ampR Construct size (kb): 2.895999908447266 Features: insert detection: lacZ marker(s): ampR promoter: T7 (phi10) promoter: lacOP replicon: f1 replicon: pMB1 MCS: EcoRI...HindIII |
| Applications： | vector permitting construction of fusion proteins vector permitting positive selection for inserts vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9, EcoRI--2.9, PstI--2.9. Cloning into the BamHI site followed by transformation into an EcoK r+ m+ host, increases the relative transformation efficiency of recombinant molecules, provided that the cloned insert is free of non-methylated EcoK sites. Vector allowing positive selection and blue/white color detection for cloned inserts. Vector constructed from pTZ19R by elimination of the EcoK restriction site in the ampR region and creation of a new EcoK site within the polylinker, containing a BamHI site as the central hexamer of the EcoK recognition sequence. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | De Backer O, et al. Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12. Gene 150: 197-198, 1994. PubMed: 7959053 |
| Shipped： | freeze-dried |