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| 产品名称： | p305 |
|---|---|
| 商品货号： | TS151119 |
| Designations： | p305 |
| Depositors： | JC Lang |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5999999046325680 Vector: p305 (plasmid) Promoters: Promoter lac Construction: pUC18, aph, herpes simplex virus TK Marker(s):G418R,ampR Construct size (kb): 4.599999904632568 Features: insert detection: lacZ marker(s): ampR, G418R promoter: lac replicon: pMB1 |
| Applications： | shuttle vector vector permitting construction of fusion proteins |
| Comments： | SmaI, HincII and EcoRI sites appear in both the polylinker and the aph cassette. Restriction digests of the clone give the following sizes (kb): HindIII--4.65; HincII--4.65; PstI--3.0, 1.7. An Escherichia coli/eukaryotic cell line shuttle vector containing lacZ and multiple cloning sites of pUC18 and the aph (neoR) gene under the control of herpes simplex virus tk promoter. A PvuII (position 628) of pUC18 was changed to BglII. The aph (aminoglycoside phosphotransferase) gene under the control of herpes simplex virus tk promoter was inserted at the BglII site. Aph is transcribed in the same direction as lacZ. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lang JC, et al. pUC vectors capable of conferring neomycin resistance of eukaryotic cells. Nucleic Acids Res. 16: 7736, 1988. PubMed: 2842735 Dinakar Desai, personal communication |
| Shipped： | freeze-dried |