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| 产品名称： | pRep(A) pREP |
|---|---|
| 商品货号： | TS151163 |
| Designations： | pRep(A) pREP |
| Depositors： | H Iba |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.1999998092651370 Vector: pRep(A) (plasmid) Construction: RSV (5 LTR, gag, pol, 5 env), pBR322 Marker(s):ampR Construct size (kb): 9.199999809265137 Features: marker(s): ampR other: 5 LTR replicon: pMB1 restriction site: SalI coding sequence: 5 env coding sequence: gag coding sequence: pol(derived from the Bryan strain of RSV |
| Applications： | vector for production of recombinant retrovirus |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV - 9.4; NdeI - 9.4; SalI - 9.4. The parental plasmid pSR-REP which encodes pol, derived from the Schmidt-Ruppin subgroup A strain of RSV, was described in Cell 34: 597-607, 1983. Rous sarcoma virus (RSV) derived vector (5 LTR, gag, pol and 5 env), for ligation with avian retroviral expression vectors (ATCC 87699 - 87701) to create proviral DNA capable of producing replication competent recombinant virus. The vector can be linearized with SalI for ligation to one of the RSV-derived expression vectors. The pol sequence is derived from the Bryan strain of RSV and is more efficient in virus production after transfection. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Murakami M, et al. High-level expression of exogenous genes by replication-competent retrovirus vectors with an internal ribosomal entry site. Gene 202: 23-29, 1997. PubMed: 9427541 Iba H, et al. Transforming potential and growth stimulating activity of the v-fos and c-fos genes carried by avian retrovirus vectors. Oncogene Res. 2: 121-133, 1988. PubMed: 2851123 Iba H, et al. Rous sarcoma virus variants that carry the cellular src gene instead of the viral src gene cannot transform chicken embryo fibroblasts. Proc. Natl. Acad. Sci. USA 81: 4424-4428, 1984. PubMed: 6087323 |
| Related Products： | component of:ATCC 87703 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |