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| 产品名称： | pARO190 |
|---|---|
| 商品货号： | TS151196 |
| Designations： | pARO190 |
| Depositors： | D Parke |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.4600000381469730 Vector: pARO190 (plasmid) Promoters: Promoter lac Construction: pUC19, pSUP2021 Marker(s):ampR Construct size (kb): 5.460000038146973 Features: marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | expression vector vector containing primer sites useful for sequencing vector permitting visual detection of recombinants vector useful for insertional mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--1.55, 0.9, 0.8 (doublet), 0.75, 0.7; HindIII/PvuI--3.9, 1.6; EcoRI/PvuI--3.9, 1.6; EcoRI--5.5. Mobilizable expression vector encoding the lacZalpha (lacZ) peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing. Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains. Replicates to high copy number in E. coli and Enterobacter aerogenes. Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4. This region can be transferred to another version of pUC vectors by using a MCS restriction site and the unique AlwNI site. The order of the major features in this plasmid is: lacZ/MCS - pMB1 ori - AlwNI site - bla - oriT/bom. pARO180 (ATCC 77123) and pARO190 (TS151196) differ in the orientation of the MCS in lacZ. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423 |
| Shipped： | freeze-dried |
| Shipping Information： | Freeze dried E. coli containing the plasmid |