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| 产品名称： | CRIPTO cDNA |
|---|---|
| 商品货号： | TS151404 |
| Designations： | CRIPTO cDNA |
| Species： | Homo sapiens, human |
| Depositors： | MG Persico |
| Applications： | When transcription is controlled by an RSV long terminal repeat, the cDNA clone induced foci of transformation in NIH3T3 cells. A probe from the 5 900 bp of this insert has been used to detect mRNA in mouse and human teratocarcinoma cells. |
| Vector： | Construct size (kb): 4.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: teratocarcinoma NT2D1 cell line Gene product: teratocarcinoma-derived growth factor 2 (retroposin, possibly expressed)(CRIPTO-like, epidermal growth factor-related oncogene) TDGF1 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.0; BamHI--4.7; HindIII--4.7; SalI--4.7; XbaI--4.7. The 900 bp probe can be excised by an EcoRI/EcoRV double digest. When transcription is controlled by an RSV long terminal repeat, the cDNA clone induced foci of transformation in NIH3T3 cells. Contains a complete coding sequence and an AT-rich 3 non-coding sequence of about 1200 bp. The 3 region includes an inverted Alu repeat, a non-canonical polyadenylation signal, and the ATTT(A) sequence of short-lived RNAs. A probe from the 5 900 bp of this insert has been used to detect mRNA in mouse and human teratocarcinoma cells. |
| References： | Ciccodicola A, et al. Molecular characterization of a gene of the EGF family expressed in undifferentiated human NTERA2 teratocarcinoma cells. EMBO J. 8: 1987-1991, 1989. PubMed: 2792079 Dono R, et al. Isolation and characterization of the CRIPTO autosomal gene and its X-linked related sequence. Am. J. Hum. Genet. 49: 555-565, 1991. PubMed: 1882841 |