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| 产品名称： | c8263 |
|---|---|
| 商品货号： | TS151456 |
| Designations： | c8263 |
| GenBank Number： | U00059 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 44.5 |
| Insert： | DNA: genomic Insert lengths(kb): 38.11000061035156 Gene product: DNA mismatch repair protein MSH1 |
| Insert Size (kb)： | 38.110 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.71 (vector); 6.05, 4.20, 4.19, 3.62, 2.99, 2.90, 2.33, 2.15, 2.06, 1.69, 1.53, 1.39, 1.05, 1.05, 0.80, 0.73, 0.73. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 313890-352000 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |