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| 产品名称： | J-Bir pERT87-J-Bir |
|---|---|
| 商品货号： | TS151508 |
| Designations： | J-Bir pERT87-J-Bir |
| Species： | Homo sapiens, human |
| Depositors： | LM Kunkel |
| Vector： | Construct size (kb): 6.21999979019165 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Gene product: DNA Segment, single copy (within DMD gene) DXS270 Alleles: A2, A1 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/KpnI--3.3, 1.94, 1.25; HindIII--3.45, 3.2; BamHI--4.0, 2.8; KpnI--4.36, 2.03; consistent with deposited DNA. The fragment is within the DMD locus. A HindIII/KpnI digestion yields 2 fragments--one at 1.8-1.9 and the other at 1.15. The latter detects the RFLP, the former is slightly repeated. The polymorphisms detected by this probe are contained within the DMD locus. Care should be taken to assure efficient transfer of the larger allele. |
| References： | Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877 Monaco AP, et al. Localization and cloning of Xp21 deletion breakpoints involved in muscular dystrophy. Hum. Genet. 75: 221-227, 1987. PubMed: 2881877 Louis M Kunkel, personal communication |