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微信小章| 产品名称： | Canine adenovirus 2 |
|---|---|
| 商品货号： | TS151511 |
| Classification： | Adenoviridae, Mastadenovirus, Canine adenovirus A |
| Deposited As： | Canine adenovirus |
| Agent： | Canine adenovirus 2 |
| Strain： | Toronto A 26/61 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Throat swab from a dog with laryngotracheitis |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | MDCK (ATCC CCL-34) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.xa0 End adsorption by adding virus growth medium. Incubation: 3-8 days |
| Name of Depositor： | LN Binn, Walter Reed Army Institute of Research, Washington, DC |
| Source： | Throat swab from a dog with laryngotracheitis |
| References： | Ditchfield J, et al. Association of a canine adenovirus (Toronto A 26/61) with an outbreak of laryngotracheitis (“Kennel Cough”): A Preliminary Report. Can. Vet. J. 3: 238-247.1962, PubMed: 17421510 Kapsenberg JG. Relationship of infectious canine hepatitis virus to human adenovirus. Proc. Soc. Exp. Biol. Med. 101: 611-614, 1959. PubMed: 14404448 |