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| 产品名称： | 182 |
|---|---|
| 商品货号： | TS151550 |
| Designations： | 182 |
| GenBank Number： | J05303 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 40.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pJB8 Intact vector size: 5.400 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI EcoRI Polylinker sites: Construction: HomerI Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: LGALS1 Genomic copy number: unique Gene name: lectin, galactoside-binding, soluble, 1 Contains complete coding sequence?: U Chromosome: 22; Localization: 22 q12-q13.1 Tissue: leukocyte Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 35.0 Cross references: DNA Seq. Acc.: J05303 Insert lengths(kb): 35.0 Tissue: leukocyte Gene product: lectin, galactoside-binding, soluble, 1 LGALS1 |
| Insert Size (kb)： | 35.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.2, 4.2, 3.0, 3.0, 3.0, 2.35, 2.05, 1.95, 1.9, 1.7, 1.5, 1.35, 1.25, 1.1, 0.92, 0.9, 0.85, 0.85, 0.8, 0.76, 0.72, 0.48, 0.42, 0.35; PvuII--4.6, 4.4, 4.1, 3.7, 3.7, 3.2, 2.2, 2.0, 1.9, 1.65, 1.25, 1.25, 1.0, 1.0, 0.75, 0.65, 0.6, 0.46, 0.4, 0.4; EcoRI--12.0, 8.0, 7.0, 4.5, 3.3, 1.65, 0.84; EcoRI/BamHI--8.4, 8.4, 4.5, 4.3, 3.65, 2.25, 1.85, 1.78, 1.7, 1.15, 0.8; EcoRI/PstI--4.2, 3.3, 3.0, 3.0, 2.35, 2.2, 1.75, 1.55, 1.35, 1.2, 1.15, 1.1, 1.0, 0.95, 0.95, 0.84, 0.75, 0.68 +smaller. The 4428 nt sequenced region includes the following restriction sites (nt from the 5 end): PstI--531, 642, 1011 (all intron 1), 1850 (intron 2); 3218 (intron 3); EcoRI--4211 (exon 4). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The sequence record begins at a Sp1 binding site consensus approximately 260 bp 5 to the initiator ATG. Contains the complete gene (4 exons). Introns 2 and 3 contain Alu repeats. |
| References： | Lau YF, Kan YW. Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin cluster. Proc. Natl. Acad. Sci. USA 80: 5225-5229, 1983. PubMed: 6310566 Gitt MA, Barondes SH. Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30: 82-89, 1991. PubMed: 1988031 |