微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pUH84 |
|---|---|
| 商品货号： | TS151559 |
| Designations： | pUH84 |
| Depositors： | B Henrich |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.19 Vector: pUH84 (plasmid) Marker(s):ampR Construct size (kb): 3.19 Features: insert detection: phiX174lysis marker(s): ampR replicon: pMB1 repressor gene: lacIq |
| Applications： | vector permitting positive selection for inserts |
| Comments： | The plasmid may be lost from transformants if stored as stabs or at -20C. For propagation and maintenance use strains with repressor-overproducing alleles of lacI; for cloning use strains that do not overproduce the lac repressor. A positive selection plasmid vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Henrich B, Plapp R. Use of the lysis gene of bacteriophage phiX174 for the construction of a positive selection vector. Gene 42: 345-349, 1986. PubMed: 2942443 Henrich B, et al. Lysis of Escherichia coli by induction of cloned phiX174 genes. Mol. Gen. Genet. 185: 493-497, 1982. PubMed: 6285147 |
| Shipped： | freeze-dried |