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| 产品名称： | pLF108 |
|---|---|
| 商品货号： | TS151600 |
| Designations： | pLF108 |
| GenBank Number： | M11147 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.21999979019165 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: PstI Vector end: PstI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: FTL Genomic copy number: unique Gene name: ferritin, light polypeptide Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 q13.3-q13.4 Tissue: liver Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.823 Cross references: DNA Seq. Acc.: M11147 Insert lengths(kb): 0.8230000138282776 Tissue: liver Gene product: ferritin, light polypeptide FTL |
| Insert Size (kb)： | 0.823 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI--5.0; PvuII--2.8, 2.1; NotI--5.0; HIndIII--4.5, 0.3, 0.25; PstI--4.3, 0.65, 0.25. A PstI digest excises the insert as two fragments (bp): 593, 230. The smaller fragment contains the 5 untranslated region. The insert contains the following restriction sites (bp from the 5 end): HindIII--569, 809; NcoI--450; NotI--372; PstI--224; PvuII--405; SacI--154. Contains the complete coding sequence (nt 152-679). |
| References： | Boyd D, et al. Structural and functional relationships of human ferritin H and L chains deduced from cDNA clones. J. Biol. Chem. 260: 11755-11761, 1985. PubMed: 3840162 Jim W Drysdale, personal communication |