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微信小章| 产品名称： | D15 |
|---|---|
| 商品货号： | TS151602 |
| Designations： | D15 |
| GenBank Number： | L10820 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.349999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: XhoI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: FPR1 Gene name: formyl peptide receptor 1 Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 q13.4 Tissue: HL-60 cell line Disease: promyelocytic leukemia Type of DNA: cDNA Insert 5 end: Modification: EcoRI linker Insert 3 end: Modification: XhoI linker Insert size (kb): 1.35 Cross references: DNA Seq. Acc.: L10820 Nucleotides 1-1350 of the insert correspond to nucleotides 525-552, 5370-6599 of L10820. Insert lengths(kb): 1.350000023841858 Tissue: HL-60 cell line promyelocytic leukemia Disease: promyelocytic leukemia Gene product: formyl peptide receptor 1 FPR1 |
| Insert Size (kb)： | 1.350 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; XhoI--4.3; EcoRI/XhoI--2.9, 1.4; BamHI--4.3; PstI--3.2, 1.1. The insert includes the following restriction sites (approximate kb from the 5 end): NcoI--0.25; PvuII--1.0; PstI--1.14; SacI--1.18. Lacks exon 2 in the 5 untranslated region that is present in some alternatively spliced transcripts. Begins within exon 1 and contains the complete exon 3. |
| References： | Murphy PM, et al. Sequence and organization of the human N-formyl peptide receptor-encoding gene. Gene 133: 285-290, 1993. PubMed: 8224916 Murphy PM, McDermott D. Functional expression of the human formyl peptide receptor in Xenopus oocytes requires a complementary human factor. J. Biol. Chem. 266: 12560-12567, 1991. PubMed: 1712023 Murphy PM, et al. A structural homologue of the N-formyl peptide receptor. Characterization and chromosome mapping of a peptide chemoattractant receptor family. J. Biol. Chem. 267: 7637-7643, 1992. PubMed: 1373134 |