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| 产品名称： | pKSM713 |
|---|---|
| 商品货号： | TS151613 |
| Designations： | pKSM713 |
| Depositors： | K Ippen-Ihler, S Maneewannakul |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0409998893737790 Vector: pKSM713 (phagemid) Promoters: Promoter for in vitro transcription T7lac Construction: pKSM710, pSM102 Marker(s):kanR Construct size (kb): 5.040999889373779 Features: insert detection: lacZalpha marker(s): kanR promoter for expression: lacUV5 promoter for in vitro transcription: T7lac replicon: f1 replicon: pMB1 repressor gene: lacIq MCS: BamHI...EcoRI terminator: trpA transcription terminator: T7 transcription terminator: fd transcription terminator: rrnB |
| Applications： | expression vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.5, 0.75. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.47; EcoRI--0.39; KpnI--0.36, 5.02; NcoI--0.33, 2.87; PstI--2.58, 3.25; PvuI--0.51; PvuII--0.48, 3.20, 3.56, 3.90, 3.99; SacI--0.38, 5.03; SalI--2.57. Differs from pKSM714 (ATCC 87160) only in the orientation of the pSM102 fragment containing lacIq, kanR and the trpA terminator. Expression vector. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823 |
| Shipped： | freeze-dried |