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| 产品名称： | Bacillus subtilis (Ehrenberg) Cohn |
|---|---|
| 商品货号： | TS151617 |
| Strain Designations： | BG2864 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | ΔaprE ΔnprE ΔflaA::kan |
| Type Strain： | no |
| Comments： | This host has been used for transformation and screening of gene mutant libraries. This strain was constructed by introducing the ΔflaA::kan mutation from strain PB5129 into strain BG2036. |
| Medium： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| Name of Depositor： | C Wang |
| References： | Shafikhani S, et al. Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization. BioTechniques 23: 304-310, 1997. PubMed: 9266088 |