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| 产品名称： | pSE1 |
|---|---|
| 商品货号： | TS151655 |
| Designations： | pSE1 |
| Depositors： | S Pestka |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 19.2500000000000000 Vector: pSE1 (plasmid) Construction: pYAC4DRI+Sup11,SV2NEO; Alu repeat (Blur-13)_ Marker(s):G418R,URA3,ampR,HIS3 Construct size (kb): 19.25 Features: marker(s): G418 (SV2neo) marker(s): HIS3 marker(s): URA3 marker(s): ampR other: Alu repeat sequence other: telomere replicon: ARS:TRP1 replicon: pMB1 restriction site: BamHI restriction site: EcoRI restriction site: NotI restriction site: SacI centromere: CEN4:sup11 |
| Applications： | acentric fragmentation vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--9.3, 8.2, 1.8; NotI--19.3; SstI--19.3. A Ura- yeast strain containing the desired YAC can be transformed with the 8.25 kb EcoRI-BamHI fragment of the vector, followed by selection on uracil-deficient media to form deletion variants of original clone. The NotI and SacI sites are unique and may be used to rescue the right end of the YAC. G418 resistance can also be used to select for transformed mammalian cells. pSE1 (TS151655) and pSE2 (ATCC 87313) differ only in the orientation of the Alu repeat sequence. Yeast artificial chromosome (YAC) fragmentation vector allowing generation of deletions from the right end of YAC clones. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cook JR, et al. Yeast artificial chromosome fragmentation vectors that utilize URA3 selection. Genet. Anal. Tech. Appl. 10: 109-112, 1993. PubMed: 8204303 |
| Shipped： | freeze-dried |