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微信小章| 产品名称： | plambdaI-3 |
|---|---|
| 商品货号： | TS151679 |
| Designations： | plambdaI-3 |
| Species： | Homo sapiens, human |
| Applications： | A 6 kb HindIII/BamHI fragment containing the entire gene has been used as a probe to detect informative bands between 0.82 and 0.91 of the PstI polymorphism. |
| Vector： | Construct size (kb): 9.5 |
| Insert： | DNA: genomic Insert lengths(kb): 6.0 Gene product: involucrin IVL Alleles: A2, A1 |
| Insert Size (kb)： | 6.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.5; HindIII--9.5; BamHI/HindIII--6.0, 3.3; EcoRI--9.5; PstI--4.2, 1.1, 0.78, 0.66, 0.5. Genomic clone of the entire human involucrin gene, including the 5 untranslated sequence, 2 exons and 1 intron. The coding region consists of 59 repeats of a 30 nucleotide sequence. The 5 flanking regions (to about 730 bp upstream of transcription start site), the 43 bp noncoding exons, and the 1.2 kb single introns are 81% identical in the human and ring-tailed lemur (Lemur catta). The coding region of the common involucrin gene contains 39 repeats of 10 codons. One of these repeats is missing in some individuals, giving rise to the PstI polymorphism. A 6 kb HindIII/BamHI fragment containing the entire gene has been used as a probe to detect informative bands between 0.82 and 0.91 of the PstI polymorphism. |
| References： | Eckert RL, Green H. Structure and evolution of the human involucrin gene. Cell 46: 583-589, 1986. PubMed: 2873896 Tseng H, Green H. Remodeling of the involucrin gene during primate evolution. Cell 54: 491-496, 1988. PubMed: 3401924 Simon M, et al. Absence of a single repeat from the coding region of the human involucrin gene leading to RFLP. Am. J. Hum. Genet. 45: 910-916, 1989. PubMed: 2574003 Simon M, et al. Polymorphism due to variable number of repeats in the human involucrin gene. Genomics 9: 576-580, 1991. PubMed: 1674722 |