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微信小章| 产品名称： | Rickettsia raoultii |
|---|---|
| 商品货号： | TS151682 |
| Classification： | Rickettsiaceae, Rickettsieae |
| Agent： | Rickettsia raoultii |
| Strain： | DnS14 |
| Applications： | Vector borne research |
| Positive for Mycoplasma： | Yes |
| Biosafety Level： | 3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Isolated from Dermacentor nutalli in Siberia, in 1994 |
| Product Format： | frozen |
| Storage Conditions： | xa0Store at -60°C or colder |
| Comments： | This item is a representative of a newly identified rickettsial species (Rickettsia raoultii), a gram-negative, obligately intracellular and non-motile bacterium. It is genetically identical to the type strain for this species (Khabarovsk, isolated in 2005) at all loci sequenced. The preparation available through ATCC tested positive for mycoplasma contamination (M.orale) by PCR. Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling. Bacteria visible in cells following staining with Gimenez stain |
| Effect on Host： | CPE in the form of cell rounding and sloughing of infected cells. |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Vero cells (ATCC CRL-1587) |
| Growth Conditions： | Recommendation for Infection: 5 days at 32°C and 5% CO2 in EMEM (ATCC 30-2003; with 2 mM L-glutamine) supplememted with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C |
| Name of Depositor： | Dr. P. -E. Fournier |
| Special Collection： | ATCC |
| Year of Origin： | 1994 |
| References： | Mediannikov, O., et. al. 2008. Rickettsia raoultii sp. nov., a spotted fever group rickettsia associated with Dermacentor ticks in Europe and Russia. Int. J Syst. Evol. Microbiol. Jul;58 (Pt 7):1635-39. PubMed: 18599708 Marrero, M and Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197-9. PubMed: 2645804 Rydkina, E. et. al., New Rickettsiae in ticks collected in territories of the former soviet union. Emerg. Infect. Dis. 1999. Nov-Dec;5(6):811-4. PubMed: 10603217 |