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| 产品名称： | human Id-1a phId-1a |
|---|---|
| 商品货号： | TS151683 |
| Designations： | human Id-1a phId-1a |
| GenBank Number： | S78986 |
| Species： | Homo sapiens, human |
| Depositors： | MA Israel |
| Applications： | contains sequence inhibitor of DNA binding 1, dominant negative helix-loop-helix protein Inhibitor of DNA binding 1, dominant negative, Id1 |
| Vector： | Construct size (kb): 3.900000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI NotI XmaIII SacII BstXI SacI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ID1 Gene name: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein Contains complete coding sequence?: Y Chromosome: 20; Localization: 20 q11 Tissue: U251 glioblastoma cell line Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 0.958 Cross references: DNA Seq. Acc.: S78986 Nucleotides 1-530 of the insert correspond to nucleotides 1-530 of S78986. Insert lengths(kb): 0.9580000042915344 Tissue: U251 glioblastoma cell line Gene product: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein( Inhibitor of DNA binding 1, dominant negative, Id1) ID1 Target Gene: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein |
| Insert Size (kb)： | 0.958 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.95; BglI--1.9, 1.3, 0.7; SmaI--3.5, 0.45; PvuI--2.3, 1.1, 0.5; PvuII--2.5, 1.05, 0.4. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.47, 0.48; PvuI--0.51, 0.64; PvuII--0.11; SmaI--0.41; SphI--0.49. The clones human Id-1a (TS151683/3) and human Id-1b (ATCC 99524/5) represent alternatively spliced products of the same gene. |
| References： | Zhu W, et al. Id gene expression during development and molecular cloning of the human Id-1 gene. Brain Res. Mol. Brain Res. 30: 312-326, 1995. PubMed: 7637581 |