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| 产品名称： | p414 GAL1 |
|---|---|
| 商品货号： | TS151700 |
| Designations： | p414 GAL1 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.4739999771118160 Vector: p414 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS414, GAL1 promoter Marker(s):TRP1,ampR Construct size (kb): 5.473999977111816 Features: marker(s): TRP1 marker(s): ampR promoter for expression: GAL1 MCS: XhoI...SpeI terminator: CYC1 centromere: CEN6/ARSH4 |
| Applications： | YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.8, 0.5; EcoRV--4.2, 1.5; HindIII--4.5, 1.3. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | freeze-dried |