| 产品名称: | pPCK10 pPCK-10 |
|---|---|
| 商品货号: | TS151707 |
| Designations: | pPCK10 pPCK-10 |
| Species: | Rattus norvegicus, rat |
| Depositors: | RW Hanson |
| Vector: | Construct size (kb): 7.099999904632568 |
| Insert: | DNA: cDNA Insert lengths(kb): 2.670000076293945 Tissue: kidney Gene product: phosphoenolpyruvate carboxykinase PCK1 Alleles: s, b |
| Insert Size (kb): | 2.670 |
| Media: | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--7.1; BglII--5.3, 1.8; PvuII--3.1, 2.8, 1.0, 0.2; PstI--4.4, 1.6, 1.1; EcoRI--4.3, 2.8. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.21, 1.8; PvuII--0.38, 1.47; PstI--1.58, 1.64; EcoRI--1.95. Detects a rat genomic BamHI fragment of 7.0 kb that contains the complete gene. A full-length cDNA clone. |
| References: | Yoo-Warren H, et al. Isolation and characterization of the gene coding for cytosolic phosphoenolpyruvate carboxykinse (GTP) from the rat. Proc. Natl. Acad. Sci. USA 80: 3656-3660, 1983. PubMed: 6304730 Siracusa LD, et al. Recombinant inbred strain and interspecific backcross analysis of molecular markers flanking the murine agouti coat color locus. Genetics 122: 669-679, 1989. PubMed: 2759422 |