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| 产品名称： | pAQP1 Xenopus expression vector UTR-01749, WI-9113, pCHIPev/BSII KS- |
|---|---|
| 商品货号： | TS151714 |
| Designations： | pAQP1 Xenopus expression vector UTR-01749, WI-9113, pCHIPev/BSII KS- |
| GenBank Number： | M77829 |
| Species： | Homo sapiens, human |
| Depositors： | P Agre, GM Preston |
| Applications： | construct to generate mRNA that can be expressed in Xenopus oocytes aquaporin 1 (channel-forming integral protein, 28 kD) |
| Vector： | Construct size (kb): 4.057000160217285 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS- Intact vector size: 2.961 Type of vector: phagemid Vector end: HindIII Vector end: PstI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, ->, 3-459 primer site: M13-20, -> promoter for in vitro transcription: T7, -> insert detection: lacZ, MCS: SacI...KpnI, ->, 657-759 promoter for in vitro transcription: T3, primer site: reverse, replicon: pMB1, 1032-1972 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: AQP1 Other gene symbols: CHIP28 Gene name: aquaporin 1 (channel-forming integral protein, 28 kD) Contains complete coding sequence?: Y Chromosome: 7; Localization: 7 p14; 7, 84.8799999 cR Tissue: bone marrow Type of DNA: cDNA Insert 5 end: HindIII Insert 3 end: PstI Insert size (kb): 1.114 Cross references: DNA Seq. Acc.: M77829 Nucleotides 57-904 of the insert correspond to nucleotides ~1-860 of M77829. Insert lengths(kb): 1.113999962806702 Tissue: bone marrow Gene product: aquaporin 1 (channel-forming integral protein, 28 kD) AQP1 Target Gene: aquaporin 1 (channel-forming integral protein, 28 kD) |
| Insert Size (kb)： | 1.114 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--1.5, 1.3, 1.1, 0.22; HindIII/PstI--2.8, 1.2; PvuII--2.4, 0.78, 0.6, 0.22. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.08, 0.27; PvuII--0.34, 0.53. The AQP1 coding sequence is flanked by 56 bp 5 untranslated sequence and 210 bp 3 untranslated sequence from the Xenopus beta-globin gene. Sense mRNA can be synthesized in vitro from the SmaI digested contruct using T3 RNA polymerase. Resulting mRNA can be injected into and expressed in Xenopus oocytes. |
| References： | Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991. PubMed: 1722319 Preston GM, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256: 385-387, 1992. PubMed: 1373524 Peter Agre, personal communication |