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| 产品名称： | pLDR11 WM2155 |
|---|---|
| 商品货号： | TS151905 |
| Designations： | pLDR11 WM2155 |
| Species： | Escherichia coli bacteriophage lambda |
| Depositors： | W Messer |
| Applications： | contains sequence attachment site integrating vector |
| Vector： | Size (kb): 4.1
Vector: pLDR11 (plasmid)
Construction: pT7-7, lambda attP, pBR322 tet
Marker(s): ampR,tetR
Construct size (kb): 4.1
Features: marker(s): ampR, tetR replicon: pMB1 |
| Insert： | DNA: genomic
Insert lengths(kb): 0.493
Gene product: attachment site attP
Target Gene: attachment site |
| Insert Size (kb)： | 0.493 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | Component of: ATCC 77371 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/BglII--3.6, 0.5; BamHI--2.2, 1.9; XbaI--4.1; NotI--2.3, 1.8. Vector containing the attP sequence for integrating DNA into the lambda attachment site attB. Requires the lambda integrase (int) such as that provided by pLDR8 (ATCC 77357). When a recombinant construct is digested with NotI, the origin is separated from the insert. Each fragment has a distinct selectable marker. After religation to form origin-free circles, the products are transformed into bacteria with pLDR8. Expression of int in pLDR8 is regulated by lambda PR, by the temperature sensitive cI857 repressor. Replication of pLDR8 is also temperature sensitive. Therefore, at 30C replication of the plasmid is normal and int is not expressed. At 42C, int is expressed but pLDR8 is no longer replicated, resulting in loss of int after a few cell generations. The order of the major features in this plasmid is: NotI - ampR - ClaI/MCS/EcoRI - attP - NotI - pMB1 ori - tetR. |
| References： | Diederich L, et al. New cloning vectors for integration in the lambda attachment site attB of the Escherichia coli chromosome. Plasmid 28: 14-24, 1992. PubMed: 1387714 |