微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pE1 |
|---|---|
| 商品货号： | TS152014 |
| Designations： | pE1 |
| GenBank Number： | X01750 |
| Species： | Homo sapiens, human |
| Depositors： | KM Gray, GF Saunders |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: genomic Insert lengths(kb): 0.6819999814033508 Tissue: biopsy Gene product: alpha satellite, XbaI D*Z1 |
| Insert Size (kb)： | 0.682 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--4.4, 0.68; HindIII--uncut; EcoRI--5.1. XbaI (insert) and HindIII (vector) ends filled in and blunt-end ligated so insert can be removed by XbaI digest; strongest hybridization to fragments of 680, 1020, 1360, 850, 1870 bp generated by XbaI-cut DNA. Hybridizes with all centromeric regions but Y; 64% hybridization signal at centromeres; 43% total label on D and G group chromosomes; no hybridization detected at telomeres. Sequence of pE1 differs from that in pF5 by 15 nucleotides; hybridizes with DNA from chimpanzee, gorilla, weakly with orangutan, not with gibbon, Old or New World monkeys. Hybridizes to certain fragments in HinfI and HindIII digested DNA; but little or no repeat sequence homology seen with EcoRI, KpnI, or PstI-digested DNA; XbaI repeat composed of 4 tandem repeats of 171, 170, 171, 170 bp. |
| References： | Alexandrov IA, et al. Chromosome-specific alpha satellites: two distince families on human chromosome 18. Genomics 11: 15-23, 1991. PubMed: 1765373 Gray KM, et al. Recent amplification of an alpha satellite DNA in humans. Nucleic Acids Res. 13: 521-535, 1985. PubMed: 2987800 |