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| 产品名称： | pQF60 |
|---|---|
| 商品货号： | TS152092 |
| Designations： | pQF60 |
| Depositors： | M Farinha |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JM109 |
| Vector Information： | Size (kb): 5.8000001907348630 DESCRIPTION OF VECTOR: Intact vector size: 5.800 Type of vector: plasmid Cloning sites: SstI KpnI SmaI BamHI XbaI AatII mLuI NcoI BglII XhoI StuI SphI HindIII Polylinker sites: EcoRI SstI KpnI SmaI BamHI XbaI SalI AatII mLuI NcoI BglII XhoI StuI PstI SphI HIndIII Construction: pCB267, pQF40 Host range: broad host range; Escherichia coli Features (with orientation and position when available): insert detection: phoA marker(s): ampR promoter: none replicon: pMB1, pRO1614 (1600) terminator: T1 Vector: pQF60 (plasmid) Promoters: Promoter none Construction: pCB267, pQF40 Marker(s):ampR Construct size (kb): 5.800000190734863 Features: insert detection: phoA marker(s): ampR promoter: none replicon: pMB1, pRO1614 (1600) terminator: T1 |
| Applications： | promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--5.8; BamHI--5.8; EcoRI--4.6, 1.2; XbaI--5.8. Promoter-cloning shuttle vector permitting visual detection of activity. Uses the promoterless alkaline phosphatase gene (phoA) as the reporter group. Translational stop codons in all reading frames are located between the MCS and the translational initiation codon of the reporter gene. The order of the major features in this plasmid is: ori(pMB1) - ori(1600) - ampR - terminator - multiple cloning site - phoA. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Farinha MA, Kropinski AM. Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters. J. Bacteriol. 172: 3496-3499, 1990. PubMed: 2111810 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |