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微信小章| 产品名称： | pV10RB TRGV10, VgammaIII |
|---|---|
| 商品货号： | TS152183 |
| Designations： | pV10RB TRGV10, VgammaIII |
| GenBank Number： | X07206 |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc |
| Vector： | Construct size (kb): 3.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 0.4000000059604645 Gene product: T cell receptor, gamma, variable region V10 TCRGV10 |
| Insert Size (kb)： | 0.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.0; EcoRI/HindIII--2.6, 0.4; EcoRI--3.0; XbaI--3.0, PstI--3.0. TCRGV10 is the unique member of the V gamma III gene subgroup. The 5 end of the gene is available as pV10PR (ATCC 59598, 59599). Subcloned from lambdaJM15. Contains the 3 region of TCRGV10. The BamHI site was not reconstituted during cloning. |
| References： | Huck S, et al. Variable region genes in the human T-cell rearranging gamma (TRG) locus: V-J junction and homology with the mouse genes. EMBO J. 7: 719-726, 1988. PubMed: 2969332 Lefranc MP, et al. Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur. J. Immunol. 19: 989-994, 1989. PubMed: 2526744 Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738 Forster A, et al. New subgroups in the human T cell rearranging Vgamma gene locus. EMBO J. 6: 1945-1950, 1987. PubMed: 2820713 |