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| 产品名称： | pCMV8 |
|---|---|
| 商品货号： | TS152226 |
| Designations： | pCMV8 |
| Depositors： | DL Davis |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.9120001792907720 DESCRIPTION OF VECTOR: Intact vector size: 4.912 Type of vector: phagemid Cloning sites: PstI SalI XbaI BamHI SmaI Polylinker sites: PstI SalI XbaI BamHI SmaI Construction: pTZ18R, bacteriophage f1, CMV promoter Features (with orientation and position when available): MCS: PstI...SmaI transcription terminator: hGH enhancer: SV40 marker(s): ampR, replicon: f1, promoter: CMV, -> Vector: pCMV8 (phagemid) Promoters: Promoter CMV Construction: pTZ18R, bacteriophage f1, CMV promoter Marker(s):ampR Construct size (kb): 4.912000179290772 Features: marker(s): ampR promoter: CMV replicon: f1 MCS: PstI...SmaI transcription terminator: hGH enhancer: SV40 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.9; SalI--4.9; XbaI--4.9. Should be grown in media containing ampicillin at a final concentration of 1 mg/ml to improve plasmid yields. May be used in essentially all cultured cells in transient transfection assays, to establish permanent cell lines and to construct transgenic mice. The pCMV7 plasmid contains a hybrid intron, while the pCMV8 plasmid contains both a hybrid intron and a 5 untranslated segment corresponding to the adenovirus major late tripartite leader sequence. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Andersson S, et al. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |