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| 产品名称： | lambdaepsilon10EZZ |
|---|---|
| 商品货号： | TS152241 |
| Designations： | lambdaepsilon10EZZ |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc, TH Rabbitts |
| Vector： | Construct size (kb): 44.0 |
| Insert： | DNA: genomic Insert lengths(kb): 14.39999961853027 Tissue: B lymphocyte (EBV transformed) Gene product: immunoglobulin epsilon IGHE |
| Insert Size (kb)： | 14.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--20.0, 11.0, 9.2, 3.8; SacI--20.0, 9.2, 3.8, 2.9, 2.3, 1.8, 1.2, 1.0, 0.65, 0.58, 0.52; XhoI--20.0, 14.0, 9.2; SmaI--20.0, 5.8, 3.7, 3.5, 1.25, 0.90, 0.84, 0.76; PvuI--20.0, 12.0, 8.2, 5.8; HindIII--23.0, 9.2, 3.9, 0.90, 0.65. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--3.9, 4.8, 5.5; BamHI--7.2, 10. Cloned from DNA isolated from individual II-4 of the TOU family. A 3.9 kb HindIII fragment from the 5 end of this insert, pH3.9EZZ, has been deposited independently as ATCC 61230, 61231. The 5 HindIII site is vector-derived. A 2.8 kb BamHI fragment contains the exons encoding the epsilon constant region. |
| References： | Keyeux G, et al. A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination. Genomics 5: 431-441, 1989. PubMed: 2613231 |