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| 产品名称： | D-157 |
|---|---|
| 商品货号： | TS152281 |
| Designations： | D-157 |
| GenBank Number： | M85168 |
| Species： | Homo sapiens, human |
| Depositors： | Y Chen |
| Vector： | Construct size (kb): 4.760000228881836 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-3Zf(+) Intact vector size: 3.199 Type of vector: phagemid Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII-SP6 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: AGL Genomic copy number: unique Gene name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) Contains complete coding sequence?: U Chromosome: 1; Localization: 1 p21 Tissue: muscle Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.563 Cross references: DNA Seq. Acc.: M85168 Insert lengths(kb): 1.562999963760376 Tissue: muscle Gene product: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) AGL |
| Insert Size (kb)： | 1.563 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 1.0, 0.5; HindIII--3.2, 1.1, 0.4; SacI/PstI--4.7; BamHI--4.0, 0.6; BglII--4.7; XbaI--4.55, 0.25. The insert contains the following restriction sites (kb from the 5 end): BamHI--0.84; BglII--0.72; EcoRI--1.42; HindIII--0.39; NcoI--0.27, 0.36; XbaI--1.28. Corresponds to nt 954-2490 of the published sequence, containing part of the coding region. |
| References： | Yang BZ, et al. Molecular cloning and nucleotide sequence of cDNA encoding human muscle glycogen debranching enzyme. J. Biol. Chem. 267: 9294-9299, 1992. PubMed: 1374391 Yuan-Tsong Chen, personal communication |