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| 产品名称： | Avian reovirus type 3 |
|---|---|
| 商品货号： | TS152297 |
| Classification： | Reoviridae, Reovirus |
| Agent： | Avian reovirus type 3 |
| Strain： | 59 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Intestine from chicken with cloacal pasting |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Deposited as Avian reovirus type 3. Pathogenic for newborn mice. |
| Effect on Host： | CPE, syncytia formation xa0CPE, syncytia formation |
| Recommended Host： | Primary chicken embryo fibroblasts Production Host: xa0Primary CEF |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2 to 7 days in a humidified 5% CO2 atmosphere |
| Name of Depositor： | BL Patel, University of Minnesota, College of Veterinary Medicine |
| Source： | Intestine from chicken with cloacal pasting |
| References： | Deshmukh DR, Pomeroy BS. Avian reoviruses. I. Isolation and serological characterization. Avian Dis. 13: 239-243, 1969. PubMed: 4307737 |