微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pCMVbeta |
|---|---|
| 商品货号： | TS152325 |
| Designations： | pCMVbeta |
| Depositors： | GR Macgregor |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.1640000343322750 Vector: pCMVbeta (plasmid) Promoters: Promoter CMV immediate early Construction: pUC19, pC4AUG beta-gal, pCM5029, pL1 Marker(s):ampR Construct size (kb): 7.164000034332275 Features: eukaryotic promoter/expression cassette: CMV promoter/enhancer SV40 splice signal eukaryotic reporter gene cassette: lacZ marker(s): ampR promoter: CMV immediate early replicon: pMB1 reporter group: lacZ terminator: SV40 late enhancer: CMV immediate early |
| Applications： | contains easily purifiable cassette(s) for construction expression vector reporter construct vector permitting visual detection of activity in colonies |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2; NotI/EcoRI--3.54, 2.9, 0.9. Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. The lacZ gene may be excised by NotI and replaced with alternative sequences. The order of the major features in this plasmid is: pUC19 - EcoRI - PstI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - PstI - HindIII - pUC19. |
| Media： | ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524 |
| Shipped： | frozen |
| Shipping Information： | Freeze dried E. coli containing the plasmid |