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| 产品名称： | pUMSV0CAT |
|---|---|
| 商品货号： | TS152377 |
| Designations： | pUMSV0CAT |
| Depositors： | K Kurachi |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5000000000000000 Vector: pUMSV0CAT (plasmid) Promoters: Promoter none Construction: pSV0cat, UMS of c-mos Marker(s):ampR,cmlS Construct size (kb): 5.5 Features: insert detection: CAT marker(s): ampR, cmlS promoter: none replicon: pMB1 |
| Applications： | promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.0; SmaI--5.7. The UMS sequences reduce background CAT transcription from the vector to levels approaching mock-transfected cells. A shuttle vector for assessing weak transcriptional activity of a cloned promoter using a CAT reporter gene. A 1023 bp SacI/XbaI fragment containing the poly(A) signal of mouse c-mos (UMS) was cloned into the NdeI site of pSV0cat. The NdeI site is 56 bp upstream of the SmaI cloning site. Constructed by J-P. Salier and K. Kurachi. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Salier JP, Kurachi K. A CAT expression vector with virtually no background: pUMSV0CAT. BioTechniques 7: 30-31, 1989. PubMed: 2629830 |
| Shipped： | freeze-dried |