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| 产品名称： | beta-tubulin lambdaEMBL3-Bgl-Pc11.4 |
|---|---|
| 商品货号： | TS152379 |
| Designations： | beta-tubulin lambdaEMBL3-Bgl-Pc11.4 |
| GenBank Number： | L05466 |
| Species： | Pneumocystis carinii Delanoe and Delanoe |
| Depositors： | TD Edlind |
| Vector： | Construct size (kb): 34.8 |
| Insert： | DNA: genomic Insert lengths(kb): 5.80 Gene product: tubulin, beta tubb |
| Insert Size (kb)： | 5.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI/BglII--20.0, 9.4, 7.7, 5.8, 1.3; BamHI--21.5, 12.2, 10.5; BglII-->23.0, 9.4, 7.7, 1.3; EcoRI--21.5, 12.2, 4.2, 3.25, 2.4. Cloned from a rat isolate. The sequenced portion predicts the following restriction sites (approximate kb from a 5 HindIII site 3 kb from the SalI end of the SalI/BglII fragment): BamHI--1.8; HindIII--1.45, 1.76; EcoRI--2.2, 2.4; KpnI--1.9; SacI--2.3; PvuI--1.26. A 1.1 kb HindIII/BglII fragment detects genomic restriction fragments of the following sizes (kb): BglII--5.8; HindIII--2.6; BglII/HindIII--1.1. Derived from a single copy gene that occurs on a chromosome of 485 kb. The complete gene is contained in a 5.8 kb BglII fragment of this clone (excised by a BglII/SalI digest). The GenBank record was derived from 2876 bp of that fragment. |
| References： | Edlind TD, et al. The beta-tubulin gene from rat and human isolates of Pneumocystis carinii. Mol. Microbiol. 6: 3365-3373, 1992. PubMed: 1484490 |