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| 产品名称： | 16C8m pET-3cT |
|---|---|
| 商品货号： | TS152412 |
| Designations： | 16C8m pET-3cT |
| Species： | Mus musculus, mouse |
| Depositors： | ET Cocuzzi, SE Walther |
| Applications： | in another host, produces protein tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6000000238418579 Tissue: embryo fibroblasts Gene product: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) Timp Target Gene: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) |
| Insert Size (kb)： | 0.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/NdeI--5.0, 0.6; PstI--3.9, 1.7; EcoRV--4.8, 0.55, 0.1; BamHI--5.6; MboII--1.65, 0.9, 0.75, 0.7, 0.6, 0.27, 0.1, +smaller. The insert contains the following restriction sites (bp from the 5 end): HaeI--4; EcoRV--177; PstI--304; XmnI--251. Constructed by modifying codon 23 from AAG to ATG, changing a StuI site to a NdeI site. The insert extends 49 bp 3 of the termination codon to the BamHI site. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Cocuzzi ET, et al. Expression and purification of mouse TIMP-1 from E. coli. FEBS Lett. 307: 375-378, 1992. PubMed: 1322839 Enzo T Cocuzzi, personal communication |