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产品简介
购买须知
| 产品名称： | pSE2.0 |
|---|---|
| 商品货号： | TS152441 |
| Designations： | pSE2.0 |
| Species： | Homo sapiens, human |
| Applications： | Has been used successfully to detect transcripts in Northern blots. |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: genomic Insert lengths(kb): 2.0 Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) RRM1 Alleles: A2, A2, A1 |
| Insert Size (kb)： | 2.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert does not contain EcoRI, KpnI, SacI or BamHI sites. The SacI and XhoI sites from the multiple cloning site of the original lambda vector GEM-11 were not removed and are there. The insert contains a HindIII site 1.3 kb from the EcoRI end and another HindIII site 0.1 kb from the SacI end. Restriction digests of the clone give the following sizes (kb): EcoRI/SacI--3.0, 2.0; HindIII--4.4, 0.6; PstI--uncut; BamHI--5.0; XhoI--3.0, 2.0. Detects a genomic SacI fragment of 18 kb. Requires preassociation with sheared human DNA prior to hybridization for optimal results. Has been used successfully to detect transcripts in Northern blots. |
| References： | Jennifer A Byrne, personal communication |