宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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Plasmodium falciparum Welch

货号 TS152443
中文名称 null
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产品简介
购买须知
产品名称: Plasmodium falciparum Welch
商品货号: TS152443
Strain Designations: FCR-1/FVO
Application:
Emerging infectious disease research
Vector-borne research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation: Adult human male, Vietnam, 1966 (?)
Product Format: frozen
Storage Conditions: Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain: no
Antibiotic Resistance:
Chloroquine-resistant
Comments:
Chloroquine-resistant
Continuous culture
Cultivation
Medium: ATCC® Medium 2196: Malaria medium, complete
Growth Conditions:
Temperature: 37°C
Culture System:xa0In vitro culture in human erythrocytes
Cryopreservation: Harvest and Preservation
Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.
  1. Centrifuge ring-stage culture for 5 mins. at 1000 x g in 50 ml centrifuge tube.
  2. Aspirate supernatant using sterile Pasteur pipet.
  3. Resuspend pellet gently in remaining supernatant.
  4. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  1. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.).
  2. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.xa0 xa0 xa0 xa0
Name of Depositor: W Trager
Chain of Custody:
ATCC <-- W Trager <-- Aotus trivirgatus (1976) <-- W.A. Siddiqui <-- Q.M. Griman/M.J. Meagher
References:

Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27: 743-746, 1978. PubMed: 356635

Trager W, Jensen JB. Human malaria parasites in continuous culture. Science 193: 673-675, 1976. PubMed: 781840

Jensen JB, Trager W. Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J. Parasitol. 63: 883-886, 1977. PubMed: 335035