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| 产品名称： | pQL1 |
|---|---|
| 商品货号： | TS152471 |
| Designations： | pQL1 |
| Depositors： | GJ Heidecker |
| Biosafety Level： | 1 |
| Vector Information： | Construct size (kb): 8.0 |
| Applications： | contains sequence |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.7, 0.3; BamHI--8.0; HindIII--8.0; PstI--3.5, 3.4, 0.9; SalI--4.6, 3.4. Do not incubate with carbon dioxide when using ribostamycin. The TSTE element is a 2.2 kb BamHI fragment that includes neoR from Tn5 flanked by Haemophilus-specific uptake sequences. Ribostamycin resistance is useful to select for the presence of neoR in both E. coli (40 ug/ml) and H. influenzae (15 ug/ml). Copy number is estimated at 6-13 per cell in both H. influenzae and E. coli. J.M. Musser |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Heidecker GJ, et al. Construction of an ori cassette for adapting shuttle vectors for use in Haemophilus influenzae. Gene 150: 141-144, 1994. PubMed: 7959040 Terrence L Stull, personal communication |
| Shipped： | freeze-dried |