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微信小章| 产品名称： | Dictyostelium discoideum Raper |
|---|---|
| 商品货号： | TS152514 |
| Strain Designations： | AX4 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Comments： | It was recently found this strain can no longer be maintained and provided as a axenic culture. So it contains its food source bacteria. Genome sequencing strain (Baylor College of Medicine, USA). |
| Medium： | ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X) |
| Growth Conditions： | Temperature: 25.0°C Duration: This culture contains food source bacteria, and thus it is not axenic. |
| Sequenced Data： | No DNA sequencing was performed in house on this product.
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| Name of Depositor： | WF Loomis |
| Special Collection： | Part of Eumycetozoan Project |
| Chain of Custody： | ATCC < |
| Isolation： | derived from strain AX3, ATCC 28368 |
| Cross References： | Complete Genome (GenBank) : AAFI00000000 Dictyostelium discoideum AX4, whole genome shotgun sequencing project |
| References： | Chaumont F, et al. Expression of an Arabidopsis plasma membrane aquaporin in Dictyostelium results in hypoosmotic sensitivity and developmental abnormalities. Proc. Natl. Acad. Sci. USA 94: 6202-6209, 1997. PubMed: 9177195 Knecht DA, et al. Developmental regulation of Dictyostelium discoideum actin gene fusions carried on low-copy and high-copy transformation vectors. Mol. Cell. Biol. 6: 3973-3983, 1986. PubMed: 3025622 Eichinger L, et al. The genome of the social amoeba Dictyostelium discoideum. Nature 435: 43-57, 2005. PubMed: 15875012 Glöckner G, et al. Sequence and analysis of chromosome 2 of Dictyostelium discoideum. Nature 418: 79-85, 2002. PubMed: 12097910 Kuspa A, Loomis WF. Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome. Proc. Natl. Acad. Sci. USA 93: 5562-5556, 1996. PubMed: 8643615 |