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微信小章| 产品名称： | HuCK |
|---|---|
| 商品货号： | TS152604 |
| Designations： | HuCK |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 2.5 Gene product: immunoglobulin kappa constant region IGKC Alleles: A1, A2 |
| Insert Size (kb)： | 2.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 2.7; HindIII--6.6; PstI--6.6; BamHI--6.6; PvuII--4.4, 3.2. The insert contains HaeIII, SacI (2), MboII, AccI, and HphI sites. The insert includes the complete exon for the constant region plus 5 and 3 flanking sequences. The polymorphic SacI site is located 3 to the SacI site in the coding sequence of the kappa constant region. Enzyme(s) not detecting polymorphism: ApaI, AvaII, BamHI, BclI, BglII, BstEII, EcoRI, EcoRV, HincII, HindIII, HinfI, KpnI, MboI, MspI, PstI, PvuII, TaqI, XbaI (tested on 7 unrelated Caucasians). |
| References： | Field LL, et al. SacI RFLP recognized by a human immunoglobulin kappa light chain constant region probe. Nucleic Acids Res. 15: 3942, 1987. PubMed: 2884643 Hieter PA, et al. Evolution of human immunoglobulin kappa J region genes. J. Biol. Chem. 257: 1516-1522, 1982. PubMed: 6276389 Weir L, Leder P. Structure and expression of a human subgroup II immunoglobulin kappa gene. Nucleic Acids Res. 14: 3957-3970, 1986. PubMed: 3086847 Hieter PA, et al. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell 22: 197-207, 1980. PubMed: 6775818 |