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| 产品名称： | pAIM-NEO |
|---|---|
| 商品货号： | TS152616 |
| Designations： | pAIM-NEO |
| GenBank Number： | Z22576 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein CD69 antigen (p60, early T-cell activation antigen) |
| Vector： | Construct size (kb): 7.699999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcDNA I/Neo Intact vector size: 7.000 Type of vector: phagemid Vector end: BstXI Vector end: BstXI Cloning sites: HindIII BamHI BstXI EcoRV NotI XhoI NsiI XbaI Polylinker sites: HindIII BamHI BstXI EcoRV BstXI NotI XhoI NsiI XbaI Host range: Escherichia coli Features (with orientation and position when available): marker(s): supF, G418R promoter: CMV replicon: SV40, polyoma, pMB1, M13 terminator: SV40 polyadenylation Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD69 Genomic copy number: unique Gene name: CD69 antigen (p60, early T-cell activation antigen) Contains complete coding sequence?: Y Chromosome: 12; Localization: 12 p13-p12 Tissue: lymphocyte, activated Type of DNA: cDNA Insert end: BstXI Insert end: BstXI Insert size (kb): 0.693 Cross references: DNA Seq. Acc.: Z22576 Nucleotides 1-694 of the insert correspond to nucleotides 12-705 of Z22576. Insert lengths(kb): 0.6930000185966492 Tissue: lymphocyte, activated Gene product: CD69 antigen (p60, early T-cell activation antigen) CD69 Target Gene: CD69 antigen (p60, early T-cell activation antigen) |
| Insert Size (kb)： | 0.693 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/NotI--7.7; BstXI--7.0, 0.7; HindIII--7.7; NcoI--2.45, 2.1, 1.55, 0.85 (doublet); SstI(SacI)--7.7. The insert contains the following restriction sites (approximate kb from the 5 end): SacI--0.07; NcoI--0.52; BstXI--0.52. Contains the complete coding sequence (nt 82-681 of the sequence record). Expresses the protein from the CMV promoter in mammalian cells. |
| References： | Lopez-Cabrera M, et al. Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors. J. Exp. Med. 178: 537-547, 1993. PubMed: 8340758 Francisco Sanchez-Madrid, personal communication |