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| 产品名称： | pC5HG2 HepG2C5 |
|---|---|
| 商品货号： | TS152624 |
| Designations： | pC5HG2 HepG2C5 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.710000038146973 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.713000059127808 Gene product: complement component 5 C5 |
| Insert Size (kb)： | 3.713 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Insert contains the following sites separated by (bp): (V/I)- 65- XmnI- 393- HindIII- 475- HhaI- 180- BamHI- 125- BglII- 453- AvaI- 234- HindIII- 608- BamHI- 140- EcoRI- 417- BstEII- 236- PvuII- 139- XmnI- 248- (I/V). V=vector, I=insert. Restriction digests of the clone give the following sizes (kb): XhoI--3.7, 3.2; EcoRI--6.9; HindIII--4.4, 1.5, 1.0; BamHI--3.2, 1.4, 1.1 doublet; PstI--2.8, 1.7, 1.2 doublet. Insert contains 3309 bp of pro-C5 coding sequence (alpha-chain, beta-alpha-chain junction, 100 amino acids of carboxy end of beta-chain) and 404 bp of 3-nontranslated sequence (with stop codon and poly(A) signal). There is 79% homology at the protein level and 80% at the nucleotide level between the human and murine C5. |
| References： | Threadgill DW, Womack JE. Syntenic conservation between humans and cattle: I. human chromosome 9. Genomics 8: 22-28, 1990. PubMed: 2081596 Brian F Tack, personal communication |