宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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Plasmodium relictum Grassi and Feletti

货号 TS152703
中文名称 null
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产品简介
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产品名称: Plasmodium relictum Grassi and Feletti
商品货号: TS152703
Strain Designations: 1P and 1P1
Application:
Vector borne research
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation: Mourning dove, Nebraska, 1937
Product Format: frozen
Storage Conditions: Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain: no
Growth Conditions: In-vivo cultivation in pigeons (age 3-4 d.)
Cryopreservation: Reagents
Glycerolyte medium
Glycerol, 57.00 g
NaC3H5O3 (Sodium lactate), 1.60 g
KCl, 30.00 mg
NaH2PO4, 1.38 g
Glass distilled water, 100.00 mL
Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave and store at 4°C.

Yaegers anticoagulant
Sodium citrate, 1.33 g
Citric acid, 0.47 g
Dextrose, 3.00 g
Sodium heparin, 0.20 g
Glass distilled water, 100.00 mL
Filter sterilize.

Harvest and Preservation
Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.
  1. To harvest parasites, inject host with ketamine (0.1–0.2 mL).
  2. Exsanguinate via the wing vein using a sterile syringe and Yaegers anticoagulant, 1 volume anticoagulant to 4 volumes blood.
  3. Centrifuge blood for 5 mins. at 1800 rpm in 50 mL centrifuge tube.
  4. Aspirate supernatant using sterile Pasteur pipet.
  5. Resuspend pellet gently in remaining supernatant.
  6. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  7. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.).
  8. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor: RG Yaeger
Chain of Custody:
ATCC
Year of Origin: 1937