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| 产品名称： | p659-2 |
|---|---|
| 商品货号： | TS152719 |
| Designations： | p659-2 |
| Species： | Homo sapiens, human |
| Depositors： | HG Zachau, W Pargent |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.200000047683716 Tissue: placenta Gene product: immunoglobulin kappa variable region IGKV Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI/SmaI--3.0, 1.2; PvuII--2.5, 1.7; PvuI--3.1, 1.1; XhoI/XbaI--3.0, 1.2; KpnI/EcoRI--3.0, 1.2. Detects sequences from the Ap (between A29 and A30) and the Ad (between A13 and A14) regions. Recloned from m659-2 which had been constructed by inserting a Sau3AI fragment into the BamHI site of M13mp10. |
| References： | Lorenz W, et al. Physical map of the human immunoglobulin K locus and its implications for the mechanisms of VK-JK rearrangement. Nucleic Acids Res. 15: 9667-9676, 1987. PubMed: 3122179 Pargent W, et al. Polymorphisms and haplotypes in the human immunoglobulin kappa locus. Eur. J. Immunol. 21: 1829-1835, 1991. PubMed: 1678350 Hans G Zachau, personal communication |