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| 产品名称： | pSVi2 |
|---|---|
| 商品货号： | TS152734 |
| Designations： | pSVi2 |
| Depositors： | MP Calos |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.1999998092651370 Vector: pSVi2 (plasmid) Construction: pML, SV40, chicken TK Marker(s):ampR Construct size (kb): 7.199999809265137 Features: insert detection: lacZ marker(s): ampR replicon: pMB1, SV40 |
| Applications： | shuttle vector |
| Comments： | The plasmid readily goes to multimers and cures at a high frequency if placed in a rec+ strain. A plasmid vector which can be replicated in both Escherichia coli and COS cells. Contains Escherichia coli lacI and lacZ, chicken TK, and SV40 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Calos MP, et al. High mutation frequency in DNA transfected into mammalian cells. Proc. Natl. Acad. Sci. USA 80: 3015-3019, 1983. PubMed: 6574469 |
| Shipped： | freeze-dried |