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| 产品名称： | pHMANT H20d |
|---|---|
| 商品货号： | TS152747 |
| Designations： | pHMANT H20d |
| GenBank Number： | J02966 |
| Species： | Homo sapiens, human |
| Depositors： | DC Wallace |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.399999976158142 Tissue: skeletal muscle Gene product: adenine nucleotide translocator 1 (skeletal muscle) ANT1 |
| Insert Size (kb)： | 1.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains NcoI (2), SacII, PstI, BglII (2), HincII, and DraI sites. If the clone is digested with HindIII/PvuII, the insert is released with about 300 bp vector sequence. Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; BglII--3.7, 0.56; ClaI--4.4; PstI--3.0, 1.15; HindIII--4.4; PvuII/HindIII--2.5, 1.7; BglI/HindIII--2.9, 1.0. This contains the complete coding sequence. The initiation Met codon begins 104 nt from the 5 end in an NcoI site. There is an open reading frame of 891 nt followed by 299 nt 3 untranslated sequence and about 100 nt poly(A). At the amino acid sequence level, this clone is more similar to a bovine adenine nucleotide translocator than to a human fibroblast adenine nucleotide translocator. |
| References： | Neckelmann N, et al. cDNA sequence of a human skeletal muscle ADP/ATP translocator: lack of a leader peptide, divergence from a fibroblast translocator cDNA, and coevolution with mitochondrial DNA genes. Proc. Natl. Acad. Sci. USA 84: 7580-7584, 1987. PubMed: 2823266 Garrison JC, et al. Isolation of full-length cDNAs encoding abundant adult human skeletal muscle mRNAs. Gene 38: 177-188, 1985. PubMed: 3840762 |