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| 产品名称： | pMHZ13 |
|---|---|
| 商品货号： | TS152811 |
| Designations： | pMHZ13 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Otherwise, RFLPs observed under normal conditions of hybridization and washing. |
| Vector： | Construct size (kb): 6.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 3.299999952316284 Gene product: DNA Segment, single copy D9S13 |
| Insert Size (kb)： | 3.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HincII--uncut; PvuII--6.3; EcoRI--5.4, 0.71; BamHI--6.3; HindIII--6.3. Codominant segregation of the PstI VNTR RFLP was demonstrated in 31 3-generation families. This probe should be preassociated with excess human DNA before hybridization. Otherwise, RFLPs observed under normal conditions of hybridization and washing. MspI does not resolve this polymorphism adequately. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pMHZ13) on chromosome 9q D9S13. Nucleic Acids Res. 16: 5703, 1988. PubMed: 2898772 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 |