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| 产品名称： | OL11 |
|---|---|
| 商品货号： | TS152843 |
| Designations： | OL11 |
| Species： | Homo sapiens, human |
| Depositors： | C Wappenschmidt |
| Applications： | Only insert DNA should be used for nick translation. |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.5 Tissue: lymphocyte Gene product: DNA Segment, single copy D12S10 Alleles: A1, A2 |
| Insert Size (kb)： | 0.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.8, 0.84, 0.37; BamHI--4.1, 0.82; SalI/HindIII--3.9, 0.66, 0.39; BamHI/HindIII--4.3, 0.6; ClaI--4.9; EcoRI--5.2, 0.5. MspI identifies 2 additional polymorphisms. Only insert DNA should be used for nick translation. Codominant segregation demonstrated in 4 informative families with 22 individuals. Enzyme(s) not detecting polymorphism: BamHI, BglII, HindIII, PvuII, XbaI with a panel of 9 unrelated Caucasians. |
| References： | Wappenschmidt C, et al. Human cDNA probe (OL11) detects RFLP on chromosome 12 (D12S10). Nucleic Acids Res. 15: 2398, 1987. PubMed: 2882473 |