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微信小章| 产品名称： | CHB201 pJC201 |
|---|---|
| 商品货号： | TS152863 |
| Designations： | CHB201 pJC201 |
| GenBank Number： | J00141 |
| Species： | Homo sapiens, human |
| Depositors： | T Shimada |
| Vector： | Construct size (kb): 8.399999618530273 |
| Insert： | DNA: genomic Insert lengths(kb): 4.0 Tissue: adult spleen Gene product: dihydrofolate reductase pseudogene 1 DHFRP1 Alleles: A1, A2 |
| Insert Size (kb)： | 4.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI-5.4, 3.1; PvuII--4.8, 3.9; BamHI--6.6, 1.9; EcoRI--4.6, 4.2. Insert contains these sites in kb from 5 EcoRI site: SstI/0.2; XbaI/?; BglII/0.57; PstI/2.3; XbaI/2.4; BamHI/2.7; AvaI/?; XbaI/3.6; SstI/3.7; EcoRI/4.0. The insert includes exon 2 to exon 6 of the intronless gene in a 0.57 EcoRI/BglII fragment at the 5 end, and a poly(A) tail. The 5 portion of the insert between EcoRI and AvaI (about 2.9 kb) contains sequences homologous to the 3 end of the normal DHFR gene. The sequence is unique, but also cross-hybridizes to pseudogenes. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. PstI detects a deletion polymorphism with the A2 allele resulting from the absence of the pseudogene. EcoRI (-/4.0), BamHI (-/5.0), and BglII (6.7/3.85) also detect the polymorphism. The polymorphism can be detected with a specific 0.42 kb XbaI/EcoRI fragment of the insert. |
| References： | Anagnou NP, et al. Chromosomal organization of the human dihydrofolate reductase genes: dispersion, selective amplification, and a novel form of polymorphism. Proc. Natl. Acad. Sci. USA 81: 5170-5174, 1984. PubMed: 6089182 Anagnou NP, et al. Chromosomal localization and racial distribution of the polymorphic human dihydrofolate reductase pseudogene (DHFRP1). Am. J. Hum. Genet. 42: 345-352, 1988. PubMed: 3341383 Chen MJ, et al. Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. USA 79: 7435-7439, 1982. PubMed: 6961421 Barbara Weiffenbach, personal communication |