微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pTF7-3 plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS152923 |
| Designations： | pTF7-3 plasmid in Escherichia coli |
| Species： | Escherichia coli bacteriophage T7 |
| Depositors： | B Moss, National Institutes of Health |
| Applications： | In another host, produces protein RNA polymerase |
| Vector： | Construct information Total size of construct (kb): unknown Marker: ampR Excise insert: BamHI Vector information Vector: pGS53 Vector type: plasmid Vector size (kb): unknown Vector ends: BamHI Construction: pUC13 ; vaccinia Wyeth Promoter: vaccinia p7.5 Replicon: pMB1 Markers: ampR |
| Insert： | Insert size (kb): 2.65 Gene: gene 1, RNA polymerase Genome: bacteriophage T7 DNA: genomic DNA Contains complete coding sequence ?: Unknown Insert ends: BamHI |
| Insert Size (kb)： | 2.650 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | ATCC VR-2153 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | In Escherichia coli containing the plasmid |
| Comments： | Also available as the vaccinia recombinant, vTF7-3, ATCC® VR-2153™.
When this plasmid is transfected into vaccinia-virus infected cells, T7 RNA polymerase activity is expressed.
The vector pGS53 includes a P7.5 promoter with early and late regulatory signals upstream of BamHI and SmaI cloning sites. These are flanked by vaccinia TK sequences from the Wyeth strain to direct recombination with vaccinia virus. |
| References： | Fuerst TR, et al. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83: 8122-8126, 1986. PubMed: 3095828 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |