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| 产品名称： | pGUS1 |
|---|---|
| 商品货号： | TS152943 |
| Designations： | pGUS1 |
| Depositors： | JE McEwen, SV Marathe |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.8699998855590820 Vector: pGUS1 (phagemid) Construction: pRAJ260, pRS316 Marker(s):URA3,ampR Construct size (kb): 6.869999885559082 Features: marker(s): URA3 marker(s): ampR replicon: CEN6 ARSH4 replicon: f1 replicon: pMB1 reporter group: gusA reporter group: lacZ MCS: KpnI...HindIII transcription terminator: CYC1 |
| Applications： | promoter-cloning vector glucuronidase, beta beta-glucuronidase shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; EcoRI/HindIII--4.8, 2.4; HindIII--7.0. Promoter-cloning vector for S. cerevisiae using E. coli GusA activity as a reliable, sensitive reporter system that allows quantitative measurement of promoter activity. The 2 kb HindIII/EcoRI fragment described as the insert includes the 100 bp transcription terminator region generated by PCR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Marathe SV, McEwen JE. Vectors with the gus reporter gene for identifying and quantitating promoter regions in Saccharomyces cerevisiae. Gene 154: 105-107, 1995. PubMed: 7867935 |
| Shipped： | freeze-dried |